dc.contributor.author | Terkawi, M Alaa | |
dc.contributor.author | Aboge, G | |
dc.contributor.author | Jia, H | |
dc.contributor.author | Goo, Y-K | |
dc.contributor.author | Ooka, H | |
dc.contributor.author | Yamagishi, J | |
dc.contributor.author | Nishikawa, Y | |
dc.contributor.author | Yokoyama, N | |
dc.contributor.author | Igarashi, I | |
dc.contributor.author | Kawazu, S-I | |
dc.contributor.author | Fujisaki, K | |
dc.contributor.author | Xuan, X | |
dc.date.accessioned | 2013-03-12T07:33:49Z | |
dc.date.issued | 2009 | |
dc.identifier.citation | Parasite Immunology, 2009,31, 328–340 | en |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/13430 | |
dc.description.abstract | Serological immune screening was used to identify a gene encoding heat shock protein-70 from Babesia gibsoni (BgHSP-70) that showed high homology with HSP-70s from other apicomplexan parasites. This gene corresponded to a full-length cDNA containing an open reading frame of 1968 bp predicted to result in a 70-kDa mature protein consisting of 656 amino acids. Analysis of the expression levels
of BgHSP-70 indicated elevated transcription from cultured parasites incubated at 40C for 1 h, but not at 30C. Interestingly, antiserum raised against recombinant BgHSP-70 protein reacted specifically not only with a 70-kDa protein of B. gibsoni but also with a corresponding native protein of B. microti (BmHSP-70), indicating the high degree of conservation of this protein. The BmHSP-70 gene was then isolated and characterized and the immunoprotective properties of recombinant BgHSP-70 (rBgHSP-70) and rBmHSP-70 were compared in vitro and in vivo. Both proteins had potent mitogenic effects on murine and canine mononuclear cells as evidenced by high proliferative responses and IFN-c production
after stimulation. Immunization regimes in BALB⁄c and C57BL⁄6 mice using rBgHSP-70 and rBmHSP-70 elicited high antibody levels, with concurrent significant reductions in peripheral parasitaemias. Taken together, these results emphasize the potential of HSP-70s as a molecular adjuvant vaccine | en |
dc.language.iso | en | en |
dc.subject | Babesia gibsoni | en |
dc.subject | Babesia microti | en |
dc.subject | Heat shock | en |
dc.subject | Protein-70 | en |
dc.subject | Immunization | en |
dc.title | Molecular and immunological characterization of Babesia gibsoni | en |
dc.type | Article | en |
local.publisher | Department of Public Health, Pharmacology and Toxicology | en |