dc.contributor.author | Gitao, C G | |
dc.date.accessioned | 2013-03-15T08:18:18Z | |
dc.date.issued | 1993 | |
dc.identifier.citation | Rev. sci. tech. Off. int. Epiz., 1993,12 (2), 639-645 | en |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/14000 | |
dc.description.abstract | The breeding of camels (Camelus dromedarius) is especially
important in arid and semi-arid areas of Africa, where drought and famine
frequently occur. A number of diseases which impair camel production have
recently been described, including dermatophilosis (caused by Dermatophilus
congolensis). However, it is not possible to determine the prevalence of
infection from clinical cases alone.
An enzyme-linked immunosorbent assay has therefore been developed to
determine the epidemiological prevalence of D. congolensis infection in sera of
camels. Whole-cell antigen was used on microplates and the test serum was
added. Horseradish peroxidase-conjugated sheep antibodies against heavy and
light chains of camel immunoglobulin (Ig)G were then added, followed by
substrate. The test was used to trace the antibody profile of twelve
experimentally-infected camels. Peak antibody levels in serum occurred within
twenty-one days following infection.
It is planned to use this test to determine the epidemiological prevalence of
D. congolensis infection in camels reared in a pastoral area of Kenya. | en |
dc.language.iso | en | en |
dc.subject | Camel | en |
dc.subject | Dermatophilosis | en |
dc.subject | ELISA - Experimental infection. | en |
dc.title | An enzyme-linked immunosorbent assay for the epidemiological survey of Dermatophilus congolensis infection in camels (Camelus dromedarius) | en |
dc.type | Article | en |