dc.contributor.author | Mwirigi, Martin K | |
dc.date.accessioned | 2013-03-18T08:46:58Z | |
dc.date.issued | 2010 | |
dc.identifier.citation | Master of Science in Applied Parasitology | en |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/14398 | |
dc.description.abstract | Mycoplasma mycoides subsp. mycoides is the etiological agent of contagious bovine
pleuropneumonia (CBPP). The disease is a significant constraint to cattle production
throughout most of Sub-Saharan Africa. The contribution of cytoadherence and molecular
factors involved in pathogenesis of M mycoides subsp. mycoides is poorly understood. This
study aimed at providing data about cytoadherence of M mycoides subsp. mycoides on
primary cultured bovine bronchus epithelial (BBE) cells and compared adherence rates to
different cell lines using flow cytometry. In this assay, M mycoides subsp. mycoides was
grown in "pleuropneumonia - like organism" (PPLO) medium and incubated with BBE cells
at 37°C. The BBE cells were then stained with polyclonal antibodies to mycoplasma shown
to adhere specifically to the BBE cells. The binding was dependent on both the concentration
of the mycoplasmas and time and up to 70-99% of epithelial cells were positive. The
specificity of mycoplasma adherence was examined using three different cell lines; bovine
skin fibroblast (BY 122 ISF pSV neo), bovine testicular endothelial cells (282 BTVE pSV
neo and baby hamster kidney (BHK) cells. Adherence rate of the three cell lines were
significantly lower than that of bovine bronchus epithelial cells. Inhibition of adherence was
observed upon pre-incubation of mycoplasma with a monoclonal antibody against capsular
polysaccharide (PK-2). Monoclonal antibody against a surface protein L-a-glycerophosphate
oxidase (GlpO) failed to inhibit significantly the adherence to BBE cells. Proteolysis of the
BBE cells surface using proteinase K partially inhibited binding of the M mycoides subsp.
mycoides to the BBE cells. Treatment of the BBE cells using glycosidase F to cleave the
carbohydrate on the surface did not show significant inhibition. The significant reduction of
adherence rate by PK-2 directed against an epitope on capsular polysaccharide located on the
surface of M mycoides subsp. mycoides, suggests involvement of mycoplasma carbohydrates
in adherence to the primary culture of BBE cells. The results also indicate that at least some
proteins on the BBE cell surface are involved inM mycoides subsp. mycoides adherence and
may be a receptor for a mycoplasma ligand. | en |
dc.description.sponsorship | University of Nairobi | en |
dc.language.iso | en | en |
dc.publisher | University of Nairobi | en |
dc.subject | Mycoplasma Mycoides Subsp. Mycoides | en |
dc.subject | Host Cells | en |
dc.subject | Parasites | en |
dc.title | Determination of the specificity and nature of Mycoplasma Mycoides Subsp. Mycoides adherence to heir Host Cells | en |
dc.type | Thesis | en |
local.embargo.terms | 6 months | en |
local.publisher | School of Biological Sciences | en |