High-Frequency Regeneration of the Drought-Tolerant Tree Melia volkensii Gurke Using Low-Cost Agrochemical Thidiazuron
Date
2012-10Author
Mulanda, ES
Adero, MO
Amugune, NO
Akunda, E
Kinyamario, JI
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Melia volkensii Gurke is a drought-tolerant tree native to East Africa’s arid and semiarid lands (ASALs), with vast but underutilized
potential for agroforestry and sustainable livelihoods in the ASALs. Its cultivation is limited by difficulties in propagation via
conventional means. Full exploitation of the ability of thidiazuron (TDZ) to elicit regeneration in plant tissue cultures, as sole
plant growth regulator (PGR), is hampered by high costs. This study tested the effectiveness of a low-cost agrochemical TDZ for
in vitro propagation of M. volkensii. Zygotic embryos from mature seeds were cultured on Gamborg’s B5 medium containing 0 to
4mg/L of agrochemical TDZ from Kingtai Chemicals Co.,Ltd., China. Callus induction frequency was 96.67 to 100%. Significantly
large callus fresh mass was produced at 0.05mg/L TDZ concentration (ANOVA, P < 0.001). The effect of TDZ on embryogenicity
was significant over certain ranges of concentrations (Anova, P < 0.001). Multiple somatic embryos developed within 14 days of
subculture to hormone-free B5 medium. Somatic embryos developed into microshoots which elongated when transferred to 1/2
MS medium supplemented with 0.1mg/L 6-benzylaminopurine plus 10% coconut water. The Kingtai-TDZ showed a high potency
and suitability for use in M. volkensii tissue culture
URI
http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/14844http://www.ncbi.nlm.nih.gov/pubmed/23227344
Citation
Biotechnology Research International Volume 2012, Article ID 818472, 5 pages doi:10.1155/2012/818472Publisher
Hindawi Publishing Corporation School of biological sciences
Subject
High-Frequency RegenerationDrought-Tolerant Tree Melia volkensii Gurke
Low-Cost Agrochemical Thidiazuron