Infectivity, pseudorecombination and mutagenesis of Kenyan cassava mosaic begomoviruses
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Date
2007Author
Bull, Simon, E.
Briddon, Rob, W.
Sserubombwe, William, S.
Ngugi, Kahiu
Markham, Peter, G.
Stanley, John
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Cloned DNA-A and DNA-B components of Kenyan isolates of East African cassava mosaic virus
(EACMV, EACMV-UG and EACMV-KE2), East African cassava mosaic Kenya virus (EACMKV)
and East African cassava mosaic Zanzibar virus (EACMZV) are shown to be infectious in cassava.
EACMV and EACMKV genomic components have the same iteron sequence (GGGGG) and can
form viable pseudorecombinants, while EACMZV components have a different sequence
(GGAGA) and are incompatible with EACMV and EACMKV. Mutagenesis of EACMZV has
demonstrated that open reading frames (ORFs) AV1 (encoding the coat protein), AV2 and AC4
are not essential for a symptomatic infection of cassava, although mutants of both ORF AV1 and
AV2 produce attenuated symptoms in this host. Furthermore, ORF AV1 and AV2 mutants
were compromised for coat protein production, suggesting a close structural and/or functional
relationship between these coding regions or their protein products.
URI
http://vir.sgmjournals.org/content/88/5/1624.longhttp://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/14926
Citation
Journal of General Virology (2007), 88Publisher
Department of plant Science and crop protection, University of Nairobi
Description
Journal article