| dc.description.abstract | presence of head blight-causing Fusarium species. Fungal contaminationwas determined by isolation on
agar media, while mycotoxin analysis was by direct competitive enzyme-linked immunosorbent assay
(ELISA). The wheat grain samples were highly contaminated with fungi, especially Epicoccum, Alternaria
and Fusarium species. The mean Fusarium infection rate varied from 13% to 18%, with the major head
blight-causing species being Fusarium poae, Fusarium graminearum, Fusarium equiseti and Fusarium
avenaceum. F. graminearum isolates were found to be highly virulent (79% disease severity) and
significantly reduced kernel weight. Most grain samples were contaminated with mycotoxins, with a
mean incidence rate of up to 75% for deoxynivalenol (DON) and 86% for T-2 toxin. Other mycotoxins
detected were zearalenone and aflatoxin B1. Co-occurrence of DON, T-2 toxin and zearalenone was
found in up to 35% of the samples. The results suggested the presence of Fusarium head blight and
associated mycotoxins in Kenya. The presence of several mycotoxins, even at such low levels, could pose
chronic adverse health effects to human and livestock fed on the contaminated wheat products. | en |
