Abstract
Pyrethrins have been analysed by several analytical technique e.g. HPLC, GLC TLC, colorimetric, polarographic, etc. This work report on the simultaneous quantitative HPLC analysis of pyrerthrins and pyrethrosin using an OD C1 HPLC column and aqueous methanol 90:10: I ) as the eluent.
Analysis of a mixture of standards gave three peaks corresponding to pyrethrosin retention time 2. 0 minutes)· pyrethrins II (retention time 4.00 minutes and pyrethrins I (retention 6.00 minutes).
Stati tical analy i of the data obtained revealed that there wa no correlation between pyrethrins and pyrethrosin concentration. hi work how that the commercial pyrethrum extract (pale extract) ha a er. 10\ ratio of pyrethrin to pyrethrins (0.50%: 18 .10%) . It also how that among t the clones rudied, clone 107 is the best due to its high pyrethrin content 2.23 %) and i relatively 10\ pyrethrin content 1. 73 %). Petal have a high concentration of pyrethrosin (2. 05% to 3. 38% and very low pyrethrin concentration 0.07% to 0.13 %)· therefore their removal before grinding of flower and extraction could significantly reduce pyrethrosin concentration in the resulting extract without significanly affecting the pyrethrins concentration.
Publisher
Depatment of Chemistry, University of Nairobi
