Detection Of Bovine Viral Diarrhoea Virus. By Nucleic Acid Hybridization
Abstract
This investigation involved the use of the p80 gene of bovine viral diarrhoea
virus (BVDV) as a probe for detection of BVDV by in situ and slot blot hybridization.
The probe was labelled with digoxigenin and reactions were detected with antidigoxigenin
alkaline phosphatase conjugate and nitroblue tetrazolium chloride and 5-
bromo-4-chloro-3-indolyl phosphate.
Negative results were occasionally obtained when in situ hybridization was used
for detection of the cytopathic, NADL strain of BVDV cultured in Madin Darby bovine
kidney (MDBK) cells. The probe was used in slot blot hybridization tests with
noncytopathic field isolates of BVDV, passaged once in MDBK cells. Of twenty
samples positive for noncytopathic BVDV by fluorescent antibody staining, seventeen
were detected by slot blot hybridization.
Based on these results, in situ and slot blot hybridization do not appear to be
applicable as routine diagnostic tests for BVDV.
Citation
Degree Of Master Of Science, University of Nairobi, 1993Publisher
University of Nairobi The Faculty of Graduate Studies oFThe University of Guelph