Characterization of Trypanosoma Congolense stocks from the West and East African regions using molecular Karyotvping
Abstract
Trypanosoma congolense, which is responsible for most of animal trypanosomiasis
10 Africa, has a great diversity of variants. Differences occur between stocks from
different geographical regions and also between stocks within the same area. In the present
work molecular karyotyping was used to investigate the differences between stocks from
two geographical regions.
Trypanosoma congolense stocks from West Africa (Gambia, Burkina Faso and
Nigeria) and East Africa (Kenya and Uganda) were grown and cloned in irradiated mice.
The DNA of the derived clones was subjected to Orthogonal Field Alternating Gel
Electrophoresis (OF AGE) in order to investigate the genomic relationships amongst the
clones.
All the clones analysed displayed one overall chromosome pattern resembling that
of T. congolense IL Nat. 3.1 (savanna type) established in similar studies. The overall
pattern was characterised by the presence of relatively small mini- chromosomes (approx.
50-150kb), lack of medium-sized chromosomes (200-400kb), and a preponderance of large
chromosomes (400-750kb). There was no clear-cut distinction between clones from the
eastern and the western regions of Africa. Similarities in chromosome profiles were
frequently observed between clones from the same stock while distinct variations were
noted in the chromosome profiles of clones belonging to different trypanosome stocks. A
few clones derived from different trypanosome stocks exhibited similar chromosome
patterns.
Cross protection experiments, carried out in mice, demonstrated that clones
exhibiting similar chromosome profiles conferred protection against each other while those
with different profiles did not. Molecular karyo-typing would therefore seem to be a
convenient and reliable technique for identifying T. congolense serodemes.
The chromosome profiles of the clones were observed to remain stable following
chronic infection for four months in goats. The profiles of the chromosome-sized DNA
molecules in terms of the number and size also remained stable following transmission
through tsetse flies.
Citation
Master of SciencePublisher
University of Nairobi