Factors Affecting Particle Retention in Thermal Field-Flow Fractionation
Date
2002Author
Shiundu, PM
Munguti, Stephen M
Wamalwa, Ben M
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
In this paper, we report a range of factors which affect the retention of colloidal
particles in thermal field-flow fractionation (ThFFF). These results are observed among different
sizes of polystyrene (PS) latex particles suspended in both aqueous and nonaqueous liquid carriers
and very low density lipoproteins in a phosphate buffer. These factors include particle size and
chemical composition, field strength, cold-wall temperature of the channel and the nature of the
suspension medium. These results show that ThFFF can be used to fractionate colloidal particles
according to size and that for an unknown colloidal sample material, a calibration curve must be
obtained using particles of similar composition. This is necessary because the degree of retention of
the colloidal material is dependent on chemical composition of sample material as evidenced by the
chemical composition study. The potential of using ThFFF for physico-chemical characterization of
colloidal material is illustrated through the evaluation of thermal diffusion coefficient of PS
particles as a function of size, cold-wall temperature, and carrier solution composition. The
tunabiliy of the extent of retention of the colloidal particles in a ThFFF channel is illustrated by
results of the influence of field strength on retention.
Citation
Factors Affecting Particle Retention in Thermal Field-Flow Fractionation, M, PROF. SHIUNDU PAUL, M PROF. SHIUNDU PAUL, and M PROF. SHIUNDU PAUL , Bull. Chem. Soc. Ethiop., 16(1),91-101, (2002) copy at http://profiles.uonbi.ac.ke/pmshiundu/publications/factors-affecting-particle-retention-thermal-field-flow-fractionationPublisher
Department of Chemistry