Treatment of cattle with DNA-encoded Flt3L and GM-CSF prior to immunization with Theileria parva candidate vaccine antigens induces CD4 and CD8 T cell IFN-γ responses but not CTL responses
Date
2011-04Author
Mwangi, DM
Honda, Y
Graham, SP
Pelle, R
Taracha, EL
Gachanja, J
Nyanjui, JK
Bray, J
Palmern, GH
Brown, WC
Mwagi, W
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Theileria parva antigens recognized by cytotoxic T lymphocytes (CTLs) are prime vaccine candidates against East Coast fever in cattle. A strategy for enhancing induction of parasite-specific T cell responses by increasing recruitment and activation of dendritic cells (DCs) at the immunization site by administration of bovine Flt3L and GM-CSF prior to inoculation with DNA vaccine constructs and MVA boost was evaluated. Analysis of immune responses showed induction of significant T. parva-specific proliferation, and IFN-γ-secreting CD4(+) and CD8(+) T cell responses in immunized cattle. However, antigen-specific CTLs were not detected. Following lethal challenge, 5/12 immunized cattle survived by day 21, whereas all the negative controls had to be euthanized due to severe disease, indicating a protective effect of the vaccine (p<0.05). The study demonstrated the potential of this technology to elicit significant MHC class II and class I restricted IFN-γ-secreting CD4(+) and CD8(+) T cells to defined vaccine candidate antigens in a natural host, but also underscores the need to improve strategies for eliciting protective CTL responses.
URI
http://hinari-gw.who.int/whalecomwww.ncbi.nlm.nih.gov/whalecom0/pubmed/21288576http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/33180
Citation
Vet Immunol Immunopathol. 2011 Apr 15;140(3-4):244-51Publisher
University of Nairobi. Department of Veterinary Pathology and Microbiology, University of Nairobi, Kenya.