Monoclonal antibody binding to a surface-exposed epitope on Cowdria ruminantium that is conserved among eight strains.
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Date
2000-11Author
Shompole, S
Rurangirwa, FR
Wambugu, A
Sitienei, J
Mwangi, DM
Type
ArticleLanguage
enMetadata
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Abstract
Monoclonal antibodies (MAb) binding to Cowdria ruminantium elementary bodies (EB) were identified by enzyme-linked immunosorbent assay, and surface binding of one MAb (446.15) to intact EB was determined by immunofluorescence, immunogold labeling, and transmission electron microscopy. MAb 446.15 bound an antigen of approximately 43 kDa in immunoblots of eight geographically distinct strains. The MAb did not react with Ehrlichia canis antigens or uninfected bovine endothelial cell lysate and may be useful in diagnostic assays and vaccine development.
URI
http://hinari-gw.who.int/whalecomwww.ncbi.nlm.nih.gov/whalecom0/pubmed/11063511http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/33255
Citation
Clin Diagn Lab Immunol. 2000 Nov;7(6):983-6.Publisher
University of Nairobi. Department of Veterinary Pathology and Microbiology, University of Nairobi, Kenya.