High-Level Heterologous Expression of Bacillus halodurans Putative Xylanase Xyn11A (BH0899) in Kluyveromyces lacti
View/ Open
Date
2007-03Author
Wamalwa Benson Munyali.
Zhao Guangshan.
Sakka Makiko.
Shiundu Paul Mwanza.
Kimura Tetsuya.
Sakka Kazuo.
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
The putative xyn11A structural gene (BH0899) encoding a family-11 xylanase from alkaliphilic Bacillus halodurans strain C-125 was heterologously expressed in the yeast Kluyveromyces lactis CBS 1065 and secreted to a level of 156 μg/ml under selective culture conditions in shake flasks. The Xyn11A production level in shake flask cultures of K. lactis CBS 1065 was higher than that reported for other xylanase genes placed under the control of the regulated LAC4 promoter on a plasmid containing an entire sequence of pKD1 from Kluyveromyces drosophilarium. Recombinant Xyn11A was highly active over pH range from 3 to 10, with maximal activity around pH 7. The enzyme showed a specific activity of 628 U/mg-protein on birchwood xylan as substrate, but no cellulase or β-xylosidase activity.
URI
https://www.jstage.jst.go.jp/article/bbb/71/3/71_60477/_articlehttp://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/33851
http://www.ncbi.nlm.nih.gov/pubmed/17341817
Citation
Bioscience, Biotechnology, and Biochemistry Vol. 71 (2007) No. 3 P 688-693Publisher
Department of Chemistry, University of Nairobi