Production of ultrasensitive antibodies against aflatoxin B 1
Date
2001Author
Gathumbi, J.K.
Usleber, E.
Martlbauer, E.
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Aims:
To produce speciÆc antibodies against the haptenic fungal toxin aØatoxin B1
(AFB1) and
apply these antibodies in immunochemical assays for aØatoxins.
Methods and Results:
Rabbits were immunized using an AFB1
-bovine serum albumin
conjugate and serum titres determined by double-antibody enzyme immunoassay. High titres
of antibodies with very high afÆnity for AFB
1
were obtained 15 and 4 weeks after the initial
immunization and the Ærst booster immunization respectively. The antibodies were employed
in enzyme immunoassay (EIA) and immunoafÆnity chromatography (IAC) methods for
aflatoxins. With a detection limit of 15
·
8pgml)1
for AFB1
, the EIA employing these
antibodies is the most sensitive test for AFB1
described so far. In IAC columns, these
antibodies provided high binding capacity for all major aØatoxins, including AFB1
, AFB2
,
AFG1
and AFG2
Conclusions:
The antibodies described here are useful for the analysis of trace levels of
aflatoxins.
SigniÆcance and Impact of the Study:
Polyclonal antibody-based EIA and IAC methods
for aflatoxin analysis offer a suitable alternative to the more expensive monoclonal antibody-
based methodS.
URI
http://onlinelibrary.wiley.com/doi/10.1046/j.1472-765X.2001.00914.x/pdfhttp://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/34151
Citation
Letters in Applied Microbiology , 32,(5) , 349-351Publisher
The Society for Applied Microbiology Department of Veterinary Pathology and Microbiology, University of Nairobi Institute for Hygiene and Technology of Food of Animal Origin, University of Munich, Germany