Characterization of antibiotic meta bolites from actinomycete isolates
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Date
2007Author
Mukunya, D M
Mutitu, E W
Muiru, W M
Type
ArticleLanguage
enMetadata
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: Metabolites from three actinomycetes isolates coded CS35, 28P and 14P antagonistic to
Pythium
spp were
produced in shaken liquid media and centrifugation done to obtain cell free culture filtrates. The antibiotic metabolites from
each of these isolates was characterized by performing chromatography & bioautography, partial purification, determination
of minimum inhibitory concentration (MIC) and determining the effect of temperature, pH and shelve life on the stability of
these metabolites. Rf values from the chromatography work and bioautography showed that, metabolites from CS35 and
14P had only one active compound whereas metabolite from isolate 28P had 2 active compounds. The antibiotic metabolites
from isolate 28P and CS35 were stable up to a temperature of 70
0
C but were sparingly stable at 80
0
C. The stability of
antibiotic metabolite from isolate 14P was drastically reduced from a temperature of 60
0
C and above. High pH value (above
10) and low pH values (below 4.0) negatively affected the stability of the metabolites. Metabolites from isolate 14P lost
stability with time whereas metabolites from isolates CS35 and 28P maintained their activity for the entire duration (5
months) tested. These metabolites were found to be sufficiently stable under different conditions and they possess a high
potential to control the disease problems under field conditions.
Citation
African Crop Science Conference Proceedings Vol. 8. pp. 2103-2107Publisher
Department of Plant Science and Crop Protection