dc.date.accessioned | 2013-06-17T14:39:21Z | |
dc.date.available | 2013-06-17T14:39:21Z | |
dc.date.issued | 1981 | |
dc.identifier.citation | lanta Med 1981; 43(10): 179-182 DOI: 10.1055/s-2007-971496 | en |
dc.identifier.uri | https://www.thieme-connect.com/ejournals/abstract/10.1055/s-2007-971496 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/35085 | |
dc.description.abstract | Valepotriates, mainly isovaltrate and valtrate, have been separated and quantitatively estimated by reversed-phase HPLC in the leaves, flowers, stems and rhizomes of Valeriana kilimandascharica. The isovaltrate/valtrate concentration reaches a maximum of 5.89% in the leaves, 3.84% in the flowers, 3.17% in the stems and 5.15% in the rhizomes.
A µ Bondapak C18 column using MeOH-H2O mixtures as eluant is suitable for a baseline separation of isovaltrate, valtrate, acevaltrate and baldrinal at UV 254 nm in 15 min and didrovaltrate and IVHD-valtrat at UV 208 nm in 10 min. Relative standard deviation for quantitative determinations is approximately 1.5% for valepotriate contents of 1%. This method is adaptable for routine analysis of crude extracts. | en |
dc.language.iso | en | en |
dc.subject | Valeriana | en |
dc.subject | Valerianaceae | en |
dc.subject | Valepotriates | en |
dc.subject | HPLC | en |
dc.title | HPCL Separation and Quantitative Determination of Valepotriates from Valeriana kilimandascharica | en |
dc.type | Article | en |
local.publisher | Department of botany, University of Nairobi | en |