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dc.contributor.authorKaaya Godwin P.
dc.contributor.authorCasper Flyg.
dc.contributor.authorHans G Boman.
dc.identifier.citationInsect Biochemistry Volume 17, Issue 2, 1987, Pages 309–315en
dc.description.abstractInjections of live Escherichia coli into adult tsetse flies, Glossina morsitans morsitans induced an antibacterial activity in the haemolymph after a lag period of 6–18 hr. Peak activity occurred after 24–72 hr with a dose of 104 bacteria/fly. Acidic electrophoresis of immune haemolymph from G. m. morsitans followed by an antibacterial assay on the gel revealed the presence of cecropin- and attacin-like factors. The induction of antibacterial activity in tsetse was completely blocked by injection of cycloheximide, a known inhibitor of protein synthesis in eukaryotic organisms. Purified InA from Bacillus thuringiensis, a proteolytic enzyme with specificity for cecropins and attacins in haemolymph, inactivated the antibacterial activity in tesetse immune haemolymph. When tested against 10 different bacterial species, the spectrum was the same for the antibacterial activity in immune haemolymph from tsetse and Cecropiaen
dc.publisherUniversity of Nairobi.en
dc.subjectInsect immunityen
dc.subjectantibacterial proteinsen
dc.subjectGlossina morsitans morsitansen
dc.titleInsect immunity: Induction of cecropin and attacin-like antibacterial factors in the haemolymph of Glossina morsitans morsitansen
local.publisherSchool of Biological Sciencesen

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