Does ethylene play a role in thidiazuron-regulated somatic embryogenesis of geranium (Pelargonium × hortorum bailey) hypocotyl cultures?
Date
1997Author
Saxena, P K
Senaratna, T
Krishnaraj, S
Murr, D
Hutchinson, M J
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
The accumulation of ethylene in headspace of hypocotyl cultures of geranium (Pelargonium × hortorum Bailey) and its possible role in thidiazuron-mediated somatic embryogenesis was investigated. The action of ethylene as determined by various ethylene synthesis and action inhibitors was varied. Silver nitrate (AgNo3), aminoethoxyvinylglycine (AVG), and silver thiosulphate (STS) had no significant influence on the embryogenic response, while 1-methylcyclopropene (1-MCP) applied during the initial 3 d of induction or the expression phase, significantly increased the number of somatic embryos formed. Thidiazuron-treated tissues accumulated large quantities of ethylene within 6 h of culture, but the levels decreased after 12 h and reached very low levels after 3 d in culture. In the presence of acetylsalicylic acid (ASA), the levels of ethylene decreased by 20 to 50% during the first 48 h of culture. Analysis of endogenous auxin, cytokinins, and abscisic acid (ABA) indicated possible interactions of ethylene with other phytohormones during the induction of somatic embryos on geranium hypocotyl explants. Thidiazuron (10 µM) increased, while ASA decreased the levels of endogenous auxin, cytokinins, and abscisic acid during this period of induction.
URI
http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/35721https://profiles.uonbi.ac.ke/mhutchinson/publications/does-ethylene-play-role-thidiazuron-regulated-somatic-embryogenesis-geraniu
Citation
n Vitro Cellular & Developmental Biology - Plant April–June 1997, Volume 33, Issue 2, pp 136-141Publisher
Department of Plant Science and Crop Protection