Protective effect of a prime-boost strategy with plasmid DNA followed by recombinant adenovirus expressing TgAMA1 as vaccines against Toxoplasma gondii infection in mice
Date
2012Author
Yu, Longzheng
Yamagishi, Junya
Zhang, Shoufa
Jin, Chunmei
Aboge, Gabriel Oluga
Zhang, Houshuang
Zhang, Guohong
Tanaka, Tetsuya
Fujisaki, Kozo
Nishikawa, Yoshifumi
Xuan, Xuenan
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
A heterologous prime-boost strategy with priming plasmid DNA followed by recombinant virus expressing relevant antigens is known to stimulate protective immunity against intracellular parasites. In this study, we have evaluated a heterologous prime-boost strategy for immunizing mice against Toxoplasma gondii infection. Our results revealed that the prime-boost strategy using both plasmid DNA and adenoviral vector encoding TgAMA1 may stimulate both humoral and Th1/Th2 cellular immune responses specific for TgAMA1. Moreover, C57BL/6 mice immunized with the pAMA1/Ad5Null, pNull/Ad5AMA1, and pAMA1/Ad5AMA1 constructs showed survival rates of 12.5%, 37.5%, and 50%, respectively. In contrast, all the pNull/Ad5Null immunized mice died after infection with the PLK-GFP strain of T. gondii. Brain cyst burden was reduced by 23% in mice immunized with pAMA1/Ad5AMA1 compared with the pNull/Ad5AMA1 immunized mice. These results demonstrate that the heterologous DNA priming and recombinant adenovirus boost strategy may provide protective immunity against T. gondii infection.
URI
http://www.sciencedirect.com/science/article/pii/S1383576912000475http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/35917