dc.contributor.author | Cao, S | |
dc.contributor.author | Luo, Y | |
dc.contributor.author | Aboge, GO | |
dc.contributor.author | Terkawi, MA | |
dc.contributor.author | Masatani, T | |
dc.contributor.author | Suzuki, H | |
dc.contributor.author | Igarashi, I | |
dc.contributor.author | Nishikawa, Y | |
dc.contributor.author | Xuan, X | |
dc.date.accessioned | 2013-06-19T07:17:28Z | |
dc.date.available | 2013-06-19T07:17:28Z | |
dc.date.issued | 2013 | |
dc.identifier.citation | Cao S, Luo Y, Aboge GO et al (2013). Identification and characterization of an interspersed repeat antigen of Babesia microti (BmIRA). Exp Parasitol. 2013 Mar;133(3):346-52. | en |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/pubmed/23291346 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/36054 | |
dc.description.abstract | In this report, a novel gene encoding an interspersed repeat antigen from Babesia microti (BmIRA) was identified and described. The full-length cDNA containing an open reading frame of 1,947 bp was obtained by immunoscreening a B. microti cDNA expression library. The full-length of BmIRA gene was expressed as a GST fusion recombinant BmIRA (rBmIRA) in Escherichia coli. Sera of mice immunized with the rBmIRA detected a native parasite protein with a molecular mass of 76 kDa on Western blot analysis. The same protein was detected in the parasites by immunofluorescent antibody test (IFAT). An enzyme-linked immunosorbent assay (ELISA) using rBmIRA detected specific antibodies as early as 11 days post-infection in sera from a hamster experimentally infected with B. microti Gray stain (US type). Furthermore, a rapid immunochromatographic test (ICT) using rBmIRA detected specific antibodies in a hamster experimentally infected with B. microti from day 11 to at least day 180 post-infection. The results indicate the antibody response against the rBmIRA was maintained during the chronic stage of infection. On the other hand, an immunoprotective property of rBmIRA as a subunit vaccine was evaluated in hamsters against B. microti challenge, but no significant protection was observed. Our data suggest that the immunodominant antigen BmIRA could be a useful serodiagnostic antigen for screening of B. microti infection. | en |
dc.language.iso | en | en |
dc.title | Identification and characterization of an interspersed repeat antigen of Babesia microti (BmIRA) | en |
dc.type | Article | en |