dc.contributor.author | Rodriguez-Martinez, H | |
dc.contributor.author | Einarsson, S | |
dc.contributor.author | Grevle, I S | |
dc.contributor.author | Hofmo, P O | |
dc.contributor.author | Bwanga, C O | |
dc.date.accessioned | 2013-06-19T12:33:46Z | |
dc.date.available | 2013-06-19T12:33:46Z | |
dc.date.issued | 1991 | |
dc.identifier.citation | Journal of Veterinary Medicine Series A Volume 38, Issue 1-10, pages 281–286, February-December 1991 | en |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/36258 | |
dc.description.abstract | Pooled ejaculates from six fertile boars were frozen under controlled conditions in Teflon® FEP-film plastic bags (5 ml) and maxi-straws (2.5 ml) using 3 % glycerol as cryoprotectant. The percentages of both post-thaw motility and normal apical ridges were significantly higher (P< 0.001) for the bags (54.5 and 75 %) than for the maxi-straws (40.1 and 59.4 %) respectively. For evaluation of the in vivo fertilizing capacity of the frozen-thawed spermatozoa, 26 gilts were inseminated once 24 h after the first observation of standing reflex in their second oestrus, with 5 ml of semen (containing 5 billion spermatozoa) reconstituted in 80 ml of BTS from either bags or maxi-straws. Ova were recovered from the oviducts/uteri 2–4 days following insemination and examined for cleavage and sperm binding to the zona pellucida (ZP). Significantly higher rates (P < 0.02) of fertilized ova were found in the bag-inseminated (75%) than in maxi-straw inseminated gilts (63%); and similarly their ova had significantly more spermatozoa in the ZP, irrespective of whether they were fertilized or non-fertilized. This study confirmed that the plastic bags are suitable and may be used for packaging single insemination doses of deep frozen boar semen for routine A. I. work. | en |
dc.language.iso | en | en |
dc.title | In vivo Fertilizing Capacity of Deep Frozen Boar Semen Packaged in Plastic Bags and Maxi‐Straws | en |
dc.type | Article | en |
local.publisher | Department of Clinical Studies | en |