Regulation Of Steroid Synthesis And Metabolism In Isolated Binucleate Cells Of The Placentae Of Sheep And Goats
Date
1992-01Author
Wango, EO
Heap, RB
Wooding, FB
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Binucleate cells of sheep and goat fetal placentae comprise about one-fifth of the trophectodermal layer at the feto-maternal interface. When isolated and incubated in vitro they produce the steroids that are synthesized by the placenta in vivo (progesterone in sheep, 5 beta-pregnane-3 alpha,20 alpha diol in goats). This study demonstrates that progesterone synthesis in binucleate cell preparations in sheep was increased by prostaglandin (PG) E-2, nordihydroguaiaracetic acid (NDGA) and methylisobutylxanthine, but reduced by indomethacin, whereas in goats only NDGA produced any effect (an increase). None of the other compounds tested (luteinizing hormone, follicle stimulating hormone, prolactin, dibutyryl cAMP, A23187 or phorbolmyristic acetate) had any effect. Sheep binucleate cells also produced PGE-2 from arachidonic acid. These results suggest that, in sheep, products of both the cyclooxygenase (producing PGE-2) and lipoxygenase (inhibited by NDGA) pathways of arachidonic acid metabolism have regulatory roles in placental steroid synthesis, but only the lipoxygenase pathway is relevant in goats.
URI
http://www.ncbi.nlm.nih.gov/pubmed/?term=Regulation+of+steroid+synthesis+and+metabolism+in+isolated+binucleate+cells+of+the+placentae+of+sheep+and+goats.http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/38401
Citation
J Reprod Fertil. 1992 Jan;94(1):203-11.Publisher
University of Nairobi Faculty Of Veterinary Medicine