Morphological And Physiological Changes During Thidiazuron-induced Somatic Embryogenesis In Geranium (pelargonium X Hortorum Bailey) Tissue Cultures
Abstract
Somatic embryogenesis in geranium (Pelargonium x hortorum Bailey cv. 'Scarlet Orbit
Improved') was achieved by culturing hypocotyls explantson media supplemented with either thidiazuron (TDZ) or indol-3-yl-acetic acid (IAA) and 6-benzylaminopurine (BAP). The physiological relationships betweenthe morphologicalchanges that lead to complete development of somatic embryos and the endogenous levels of plant growth regulators were studied. . mz induced similar but more pronounced and earlier morphological changes in cultured tissues than did IAA and BAP, but after 21 d in culture, both treatments promoted from the formation of somatic embryos of different developmental stages that were similar to zygotic induced a higher number of somatic embryos, with similar but greater increases in theendogenous levels auxins and cytokinins, than did IAA and BAP. This may indicated that TDZ may be a more potent plant growth regulator in inducing physiological and morphological changes than combined auxin and cytokinin, during the process of somatic embryogenesis in geranium
Citation
Hutchinson, MJ. 1996. Morphological and Physiological changes during thidiazuron-induced somatic embryogenesis in geranium (Pelargonium x hortorum Bailey) tissue cultures. International Journal of Plant Science. 157:440-446.Publisher
University of Nairobi Plant Science and Crop Protection