Comparison of two ribosomal DNA-based methods for differentiating members of the Anopheles gambiae complex (Diptera: Culicidae).
| dc.contributor.author | Taylor, KA | |
| dc.contributor.author | Paskewitz, SM | |
| dc.contributor.author | Copeland, RS | |
| dc.contributor.author | Koros, J | |
| dc.contributor.author | Beach, RF | |
| dc.contributor.author | Githure, JI | |
| dc.contributor.author | Collins, FH | |
| dc.date.accessioned | 2013-06-29T09:49:46Z | |
| dc.date.available | 2013-06-29T09:49:46Z | |
| dc.date.issued | 1993-03 | |
| dc.identifier.citation | J Med Entomol. 1993 Mar;30(2):457-61. | en |
| dc.identifier.uri | http://hinari-gw.who.int/whalecomwww.ncbi.nlm.nih.gov/whalecom0/pubmed/8096250 | |
| dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/42302 | |
| dc.description.abstract | Two DNA-based methods, the restriction fragment length polymorphism (RFLP) and polymerase chain reaction (PCR), were used to identify mosquitoes of the Anopheles gambiae Giles complex collected in Kenya. Field-collected specimens of An. gambiae, An. arabiensis Patton, and An. merus Donity were tested. From a sample of 208 mosquitoes, 181 (87%) were identified by the RFLP method and 205 (99%) were identified by the PCR method. There was complete concordance between the two methods with regard to species identification. PCR assays were simpler, faster, and more reliable than RFLP assays. | en |
| dc.language.iso | en | en |
| dc.publisher | University of Nairobi. | en |
| dc.title | Comparison of two ribosomal DNA-based methods for differentiating members of the Anopheles gambiae complex (Diptera: Culicidae). | en |
| dc.type | Article | en |
| local.publisher | Department of Zoology | en |
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