Changes in the contents of intermediates of proline and carbohydrate metabolism in flight muscle of the tsetse fly Glossina morsit ans and the fleshfly Sarcophaga tibialis
Olembo, Norah K
Pearson, David J
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1. The contents of intermediates of proline metabolism, the tricarboxylate cycle and glycolysis were measured over the initial phases of flight in the thorax of the tsetse fly Glossina morsitans and the fleshfly Sarcophaga tibialis. 2. The initial rates of proline loss and alanine increase in G. morsitans were approx. twelve times those found in S. tibialis. 3. In G. morsitans there was a sharp initial decrease in the contents of glutamate, ammonia and citrate. Pyruvate content increased rapidly and those of malate and α-oxoglutarate more slowly. Similar initial changes in the contents of these intermediates were observed in S. tibialis. The total concentration of measured tricarboxylate cycle compounds did not change greatly in either insect when flight commenced. 4. Hexose monophosphate concentrations were much lower in G. morsitans than in S. tibialis, but in both insects an increase in the fructose-1,6-diphosphate/fructose-6-phosphate ratio occurred at the onset of flight. 5. The control of the tricarboxylate cycle and the proline-alanine pathway is considered. The utilisation of proline by S. tibialis during flight is discussed.
CitationInsect Biochemistry Volume 12, Issue 6, 1982, Pages 657–662
University of NairobiDepartment of Biochemistry