Analysis of Iris yellow spot virus replication in vector and non-vector thrips species
Date
2013Author
Birithia, R
Subramanian, S
Pappu, H R
Muthomi, J
Narla, R D
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Iris yellow spot virus (IYSV, genus Tospovirus) is a viral disease of bulb and seed onion crops and is transmitted by Thrips tabaci. Foliage damage of up to 75% has been reported in Kenya and Uganda. In this study, the rate of IYSV replication in the larva, pupa and adult stages of T. tabaci and other non-vector thrips species and colour forms such as Frankliniella occidentalis, F. schultzei (dark) and F. schultzei (pale) was evaluated by monitoring relative levels of nucleocapsid (N) and non-structural (NSs) proteins using N- and NSs-specific antibodies. The effect of IYSV replication on mortality of thrips was also determined. N protein levels increased in all three stages of IYSV-fed T. tabaci, indicating replication of IYSV. In IYSV-fed non-vector thrips, the increase of N protein levels in the larval stage was lower than IYSV-fed T. tabaci but higher than their healthy counterparts. The N protein levels did not increase at pupal and adult stages. NSs protein was not detected in first instar of either vector or non-vector thrips species. After a 4 h post-acquisition period, a significant increase in NSs proteins was only observed in IYSV-fed T. tabaci, clearly differentiating vectors and non-vectors of IYSV. IYSV replication did not influence the survival of the vector thrips species, T. tabaci populations or the non-vector thrips species. This study indicates the effectiveness of monitoring non-structural proteins such as NSs, compared to nucleocapsid proteins, for differentiating vectors and non-vectors of IYSV.
URI
http://onlinelibrary.wiley.com/doi/10.1111/ppa.12057/abstracthttp://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/43917
Citation
Plant Pathology, 2013Publisher
Department of Plant Science and Crop Protection, University of Nairobi
Subject
Iris yellow spot virusNon-structural protein
Nucleocapsid protein
Replication
Thrips tabaci
Description
Journal article