Selecting Rhizobium Phaseoli Strains For Use With Beans (Phaseolus Vulgaris L.) In Kenya . I. Ineffectiveness And Tolerance Of Acidity And Aluminium.
Abstract
Forty one strains of Rhizobium phaseoli were screened for the ability to multiply at high temperatures on yeast extract-mannitol agar. Most strains were tolerant of 30°C, eight strains were tolerant of 45°C and two of 47°C although the rate of multiplication was reduced at 45–47°C. The high temperature-tolerant strains were isolated from Kenyan soils and were fast-growing. Seven of the eight strains tolerant of 45–47°C lost their infectiveness after incubation at high temperature but four strains tolerant of 40°C remained infective after incubation at that temperature.
Thirty six strains were resistant to 200 μg ml−1 streptomycin sulphate and 29 strains to 200 μg ml−1 spectinomycin dihydrochloride. Eight strains were resistant to both antibiotics each at 200 μg ml−1. Two of the double-labelled antibiotic-resistant mutants lost their infectiveness onPhaseolus vulgaris. The response to acidity was unaltered and two of the mutants showed a decrease in temperature tolerance. The doublelabelled mutants were recoverable from two Kenyan soils.
URI
http://link.springer.com/article/10.1007/BF02181747http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/49984
Citation
Plant and Soil November 1988, Volume 112, Issue 1, pp 15-22Publisher
University of Nairobi Department of Land Resource Management and Agricultural Technology