Microvascular endowment in the developing chicken embryo lung
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Date
2007Author
Makanya, AN
Hlushchuk, R
Baum, O
Velinov, N
Ochs, M
Djonov, V
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
In the current study, the contribution of the major angiogenic mechanisms, sprouting and intussusception, to vascular development in the avian lung has been demonstrated. Sprouting guides the emerging vessels to form the primordial vascular plexus, which successively surrounds and encloses the parabronchi. Intussusceptive angiogenesis has an upsurge from embryonic day 15 (E15) and contributes to the remarkably rapid expansion of the capillary plexus. Increased blood flow stimulates formation of pillars (the archetype of intussusception) in rows, their subsequent fusion and concomitant delineation of slender, solitary vascular entities from the disorganized meshwork, thus crafting the organ-specific angioarchitecture. Morphometric investigations revealed that sprouting is preponderant in the early period of development with a peak at E15 but is subsequently supplanted by intussusceptive angiogenesis by the time of hatching. Quantitative RT-PCR revealed that moderate levels of basic FGF (bFGF) and VEGF-A were maintained during the sprouting phase while PDGF-B remained minimal. All three factors were elevated during the intussusceptive phase. Immunohistoreactivity for VEGF was mainly in the epithelial cells, whereas bFGF was confined to the stromal compartment. Temporospatial interplay between sprouting and intussusceptive angiogenesis fabricates a unique vascular angioarchitecture that contributes to the establishment of a highly efficient gas exchange system characteristic of the avian lung
URI
http://ajplung.physiology.org/content/292/5/L1136.full.pdf+htmlhttp://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/50312
Citation
AJP - Lung Physiol May 2007 vol. 292 no. 5 L1136-L1146Publisher
The American Physiological Society Department of Veterinary Anatomy and Physiology, University of Nairobi, Nairobi, Institute of Anatomy, University of Bern, Bern, Switzerland
Subject
Intussusceptive angiogenesisVascular patterning
Lung development
Blood-gas barrier
Sprouting angiogenesis