The Sensitivity And Specificity Of Clinical Signs, Post-mortem Findings And Isolation Of Escherichia Coli In Diagnosing Edema Disease Of Swine.
Date
2008Author
Karanja, D.N.
Ngatia, T.A.
Wabacha, J.K.
Bebora, L.C.
Type
PresentationLanguage
enMetadata
Show full item recordAbstract
Commonly used diagnost
ic techniques for edema
disease in Kenya were tested for
their
sensitivity and specificity on 84 pig carcasses submitted to Department of Veterinary
Pathology and Microbiology between June 2004 and June 2007. Clinical signs gathered from
anamnesis, post
-
mortem lesions revealed at autopsy and
E. coli
isolated from intestinal
contents and characterized using biochemical tests and polymerase chain reacti
on were
compared using receiver
-
operating characteristic analyses. A combination of clinical signs,
post
-
mortem findings and isolation of
E. coli
carrying F18 and shiga
-
toxin type II variant
genes were used as gold standard test. Forty nine (58.3%) cases w
ere diagnosed as edema
disease, based on clinical signs and post
-
mortem findings
. Of these, thirty eight (77.6%) had
variable amounts of edema in various body si
tes and clinically, twenty six
(53.1%) had
neurological signs, 18 (36.7%) were found dead, 3 (
6.1%) had swollen eyelids and 2 (4.1%)
expressed respiratory distress. Hemolytic
E. coli
carrying the tested genes were isolated
from thirty one (62.2%) of these cases. Presence of edema in various body cavities and
observation of defined clinical signs h
ad 75.3% and 57.4% sensitivity, respectively.
Considered individually, the sensitivity was 64.7% for found dead, 50% for neurological signs
and 84.4% for isolating
hemolytic
E. coli.
All had a specificity of 81.3%. The results show that
none of the diagnos
tic techniques had the expected 100% sensitivity and specificity, but
isolation of hemolytic
E. coli
may be an important screening test for suspected edema
disease cases
Citation
D.N., Karanja, Ngatia T.A., Bebora L.C, J.K. W. 2008. The sensitivity and specificity of clinical signs, post-mortem findings and isolation of Escherichia coli in diagnosing Edema disease of swine., 2008. Paper presented at the Faculty of Veterinar y Medicine Scientific Conference held in Nairobi, 2008. Proceedings page 33Publisher
u Veterinary Panthology and Microbiology