Localisation Of Newcastle Disease Viral Nucleoprotein In The Tissues Of Carrier Ducks.
Date
2008Author
Njagi, L.W.
Mbuthia, P.G.
Nyaga, P.N.
Bebora, L.C.
Michieka, J.N
Minga, U.M
Type
PresentationLanguage
enMetadata
Show full item recordAbstract
Localisation of
Newcastle disease viral nucleo
protein in the tissues of carrier ducks was
evaluated in 45 experimentally infected and 10 sentinel ducks. Ten chickens were used as
positive control bir
ds. The ducks were sacrificed serially on days 1, 4, 8 and 14
–
post
-
inoculation. Six tissues (liver, spleen, lung, cecal tonsils, kidneys and brain) were collected
from each bird, preserved in 10% neutral formalin for 24 hours, and then transferred to 70%
ethanol. Indirect alkaline phosphatase
–
antialkaline phosphatase immunoperoxidase
staining was performed to detect viral nucleoprotein. The ducks (28.9
%) had
Newcastle
disease viral nucleoprotein in their tissues. The viral nucleoprotein
s
were found in l
arge
mononuclear cells of cecal tonsils and tubular epithelial cells in the kidneys of infected
ducks. The viral antigens were located in the cytoplasm and nucleolus of the respective cells.
Liver, lungs, spleen and brain of all infected ducks did not have
detectable viral antigens.
The number of ducks with viral antigen increased with duration of infection from 22.2%,
16.7%, 33.3% and 41.7% on days 1, 4, 8 and 14 post
-
inoculation
, respectively (p<0.05).Viral
antigen intensity in cecal tonsil tissue section
s was 4, 5, and > 5 cells in 15.4%, 53.8% and
30.8%, respectively, of the infected ducks. In the kidneys, more than 5 positive cells were
recorded. Thus, in Newcastle disease virus carrier ducks, the kidneys and cecal tonsils need
to be sampled for virus i
solation besides other tissues.
Citation
Njagi L.W., Mbuthia P.G., Nyaga P.N., Bebora L.C., Michieka J.N. and Minga U.M (2008): Localisation of Newcastle disease viral nucleoprotein in the tissues of carrier ducks. Paper presented at the Faculty of Veterinary Medicine Scientific Conference held in Nairobi, 2008. Proceedings page 26 - 27Publisher
University of Nairobi Veterinary Panthology and Microbiology