Effects of enzyme complex (Allzyme SSF) and Yeast metabolites (Diamond V XPC) on performance, immune responses, Gastrointestinal morphology and Intestinal Microbiota in broiler Chicken (Gallus Domesticus)

Abstract

A study was done to determine the effects of yeast metabolites (Diamond V XPC) and enzyme complex (Allzyme SSF) on the performance of broiler chickens. 160 Abor Acre broiler chicks were fed on basal diets supplemented with either an enzyme complex of a protease, amylase, xylanase, β-glucanase, pectinase, cellulase and phyatase (0.2g/kg); yeast metabolites (1.25g/kg for broiler starter diet and 0.63g/kg for broiler finisher diet; or both. The basal diet without any feed additive served as a control. Birds were vaccinated with F-strain of Newcastle disease virus vaccine at 28 days of age and later given a booster dose one week later. One bird in each replicate (a total of four birds per dietary treatment) was slaughtered at 21 days of age (before vaccination) and at 42 days of age (after vaccination). During each slaughter, the length of rectum, caeca, ileum, duodenum, jejunum and jejuna villi were taken and recorded. Fresh gizzard, liver and bursa of Fabricius were weighed and recorded. During the last slaughter at 42 days, the abdominal fat pad was removed and weighed while fresh and the weight recorded. Performance parameters such as feed intake, weight gain, body weight, FCR and PER were recorded on 21st and 42nd day of age. Blood was collected during each slaughter and serum obtained by centrifugation to determine the immune response measured by antibody titer. Fecal materials were removed from the rectum for total bacterial counts, coliform counts and coccidian oocysts counts. There were no significant effects (P<0.05) of yeast metabolites or enzyme complex on growth performance and weight of internal organs. The treatment diets also had no significant effects (P<0.05) on bacteria count, coliforms, oocysts and on antibody titer. Broilers fed diets supplemented with yeast metabolites had longer jejunum and ceacum (P<0.05) at 42 days than those fed diets supplemented with enzyme complex. However, no significant effects of yeast metabolites and enzyme complex were found on the length of duodenum, ileum and rectum.