Specific PCR assay for a tannin-tolerant selenomonas ruminantium isolate, derived from helicase coding sequences
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Date
2004Author
Bishop, R
Obura, M
Odongo, D
Odenyo, A
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Sequences from a tannin-tolerant Selenomonas ruminantium isolate (EAT2) that hydrolyzes gallic acid were identified. Two exhibited identity to helicases with a wide phylogenetic distribution. PCR amplification by using primers from one helicase gene detected 2000 to 5000 EAT2 genome equivalents but did not amplify total gastrointestinal microbial DNA of nine other ungulate species.
URI
http://www.ncbi.nlm.nih.gov/pubmed/15128588http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/58165
Publisher
College of Biological and Physical Sciences ,Department of Biological Science