| dc.description.abstract | Schistosoma species are responsible for causing Schistosomiasis in humans. It is the
second most important parasitic infection, after malaria, mainly infecting people in the
tropics. It afflicts approximately 207 million people worldwide, 700 million are at risk
and causes 500 hundred deaths annually, 85% of the infected are from Africa and
majority are children. Praziquantel, which is the effective drug to all schistosomes does
not kill all parasites, is expensive and there is the risk of resistance to the drug. The recent
resurgence of interest in the study of antischistosomal medicinal plants of local origin is
largely based on a self-reliance philosophy. Thus, medicinal plants seem to be an
affordable and readily available local alternative source of drugs. The aim of this study
was, therefore, to evaluate the antischistosomal properties of Azadirachta indica and
Entada leptostachya extracts on Schistosoma mansoni infection as possible leads to drug
development. For each of the plants, there were two extracts used; Aqueous [Azadirachta
indica (AA) Aqueous and Entada leptostachya Aqueous(ELA)] and Methanol
[Azadirachta indica Methanol (AM) and Entada leptostachya Methanol (ELM)]. A total
of 78 mice were used in the study; 72 in the experimental groups, each with 12 mice and
6 mice were Naïve for IgG ELISA. Mice in groups of six were individually infected with
200 Schistosoma mansoni cercariae. Four weeks post infection, they were orally treated
separately with 150mg/kg of Azadirachta indica and Entada leptostachya methanol or
aqueous extracts two days apart. Another group of twelve mice were treated with
praziquantel (PZQ) as a positive control and there was the infected control (IC) group. At
the sixth week, all animals were perfused to evaluate the efficacy of the plant extracts in
the treatment of the infection. Worm recovery, pathological, and immunological (cellular
and humoral) assays were carried out to measure the antischistosomal activity of aqueous
and methanol extracts of the two plants. The results obtained showed a 15.42%
maturation of penetrant cercariae. The plant extracts showed no immunological effects on
Schistosoma mansoni infection in BALB/c mice. In both Azadirachta indica and Entada
leptostachya extracts there was no worm reduction. However, the extracts of Entada
leptostachya recorded a lower worm burden than the extracts of Azadirachta indica.
Results showed higher levels of IFN-γ responses for all plant extracts which confirmed
the severe pathology observed. There were high IL-5 levels attributed to the presence of
non-specific schistosome antigens not responsible for protection of the host against the
infection. The four extracts stimulated high IgG levels which did not confer any
protective immunity and which was not significantly different from IC (p>0.05)
especially for SWAP antigens. | en |
