To be or not to be a species: use of reproductive isolation experiments and genetic analysis to clarify the taxonomic status of two Busseola (Lepidopter a: Noctuidae) species in Kenya
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Date
2013Author
Anne-Emmanuelle, Felix
Paul-André, Calatayud
Bruno, Le R u
Claire, Capdevielle-Dulac
Ong ’amo, George
Jean-François, Silvain
Brigitte, Frérot
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Phylogenetic analysis combined with chemical ecology can contribute to the delimitation of closely related
insect species, particularly in Lepidoptera. In this study, the taxonomic status of a species in the genus Busseola
(Lepidoptera: Noctuidae) was discussed using morphological data, cross-mating experiments, sex pheromone chemistry,
field-trapping, and molecular classification. The results of the chemical ecology experiments corroborated those from the
phylogeny studies. It was concluded that several reproductive isolation components, namely host plants, geography,
pheromone emission time, pheromone blend, and post-zygotic isolation factors, led to the separation of Busseola n. sp.
from its closely related species B. segeta. Molecular data showed a strong difference between these two species, regardless
of the marker used. The new species named Busseola nairobica was morphologically described and a hypothesis about the
evolutionary history of the studied species was put forward.
Citation
International Journal of Entomology , 49:3, 345-354, 2013Publisher
Taylor & Fr ancis
Subject
Busseola fuscaBusseola segeta
Busseola nairobica
Morphology
Pheromone
Feld-trapping
Molecular phylogeny
Cytochrome b
L5 ribosomal protein gene