Transferring striga resistance genes into farmer preferred sorghum variety in Rwanda
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Date
2011-09Author
DeVilliers, Santie
Kiambi, Dan
Mutitu, Eunice
Ngugi, Kahiu
Niyibigira, Theogene
Language
enMetadata
Show full item recordAbstract
Striga hermonthica is one of the major constraints of sorghum production
world-wide and particularly in eastern province of Rwanda. In this study, 5
Striga resistance Quantitative Trait Loci (QTLs) from a resistance donor N13
line and located on SB1-01, SB1-02, SB1-05 (with 2 QTLs) and SB1-06 were
transferred into IS8193 line, a farmer preferred sorghum variety in the eastern
province of the country, The transfer was performed using Simple Sequences
Repeats (SSRs) molecular markers. Nine foreground polymorphic SSR
markers were used to identify F 1 progenies carrying Striga resistance QTLs.
From a cross between IS8193 and N13, a total amount of20 F1 progenies were
genotyped and 16 ofthem were found to have one to three introgressed Striga
resistance QTLs. The 16 selected genotypes were backcrossed to IS8193 to
produce BC1F1 generation. After BC1F1 progenies genotyping, 12 BC1F1
lines were identified to carry one to three Striga resistance QTLs and were
again backcrossed to IS8193 to produce BC2F1 genotypes. The BC2F1
generation with one to three QTL will be genotyped to produce BC3F I and also
selfed to BC2S1 to produce almost stable Striga resistant sorghum lines for
adoption by farmers in Rwanda.
Citation
Optimimization of Agricultural Value Chains for sustainable DevelopmentSponsorhip
National Council of Science and Technology, The Kenya Seed CompanyPublisher
Faculty of Agriculture, University of Nairobi
Description
Oral presentation at aGRO 2011 Biennial Conference