Lignocellulolytic Activities Of Crude Gut Extracts Of Marine Woodborers Dicyathifer Mannii And Sphaeroma Terebrans
View/ Open
Date
2006Author
Bosire, C.M.
Abubakar, L aila
Ochanda, James
Bosire, J O
Language
enMetadata
Show full item recordAbstract
Marine woodborers
have a close association with tropical mangrove plants whereby they
voraciously
consume
lignocellulose and play a role in nutrient cycling. T
hey represent a rich source of potential lignocellulo
lytic
enzymes that can be harnessed for conversion of biomass into simple sugars and other monomers for a variety of
uses. Ligninolytic enzymes find applications in bio bleaching of pulp and decolouration of textile dyes, whereas
cellulolytic and hemicellu
lolytic enzymes find applications in animal feed, manufacture of bread, bioethanol
production and xylitol production among other uses.
In this study
,
we obtained crude gut extracts from two
marine woodborers
,
Dicyathifer mannii
(Wright, 1866) and
Sphaeroma
terebrans
(Bate, 1866),
from three
sampling sites along the Kenyan coast.
Lignocellulolytic activities of the gut extracts were investigated in an
effort to seek the species with the most lignocellulolytic efficacious extracts
.
Ligninolytic activities inv
estigated
were lignin peroxidase (LiP), manganese
-
dependent peroxidase (MnP) and laccase (Lac) or monophenol
oxidase. Cellulolytic enzymes investigated were gluc
anases endoglucanase (endo
-
1
-
4
-
β
-
D
-
glucanase),
exoglucanase (1,4
-
β
-
D
-
glucan
-
cellobiohydrolase), and
β
-
D
-
gl
ucosidase or cellobiase (
β
-
D
-
glucosid
e
glucanohydrolase). Endo
-
1
-
4
-
β
-
xylanase was investigated in the hydrolysis of xylan, the chief type of
hemicellulose.
D. mannii
crude extracts
showed an appreciable Lip activity
of up to 34.65
±
0.
1
16
U/L and
endoglucanase (CMCase) activity of up to 50.7 U/ml (1 U represents
the amount of enzyme which catalyzed the
transformation of
1 micromol of
substrate
min
-
1
).
D. mannii
is implicated as a sourc
e of these enzymes for
industrial use.
URI
http://www.innspub.net/wp-content/uploads/2013/12/IJB-V3No12-p134-144.pdfhttp://hdl.handle.net/11295/73445
Citation
Abubakar, L. U., Bulimo, W. D., Mulaa, F. J., & Osir, E. O. (2006). Molecular characterization of a tsetse fly midgut proteolytic lectin that mediates differentiation of African trypanosomes. Insect biochemistry and molecular biology, 36(4), 344-352.Publisher
University of Nairobi