Browsing Journal Articles by Author "Xuan, X"
Now showing items 1-12 of 12
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Babesia gibsoni: identification, expression, localization, and serological characterization of a Babesia gibsoni 22-kDa protein
Goo, YK; Jia, H; Terkawi, MA; Aboge, GO; Yamagishi, J; Nishikawa, Y; Kim, S; Jang, HK; Fujisaki, K; Xuan, X (2009)Babesia gibsoni causes canine babesiosis. Here, we describe the identification and characterization of a novel gene, BgP22, containing an open reading frame of 621bp and encoding a 22-kDa protein from B. gibsoni, as a ... -
Characterization of a leucine aminopeptidase of Babesia gibsoni
Jia, H; Terkawi, M. A; Aboge, G. O; Goo, Y.K; Luo, Y; Li, Y; Yamagishi, J; Nishikawa, Y; Igarashi, I; Sugimoto, C; Fujisaki, K; Xuan, X (2009)Peptidases of parasitic protozoa are currently under intense investigation in order to identify novel virulence factors, drug targets, and vaccine candidates, except in Babesia. Leucine aminopeptidases in protozoa, such ... -
Epidemiological Survey of Babesia bovis and Babesia bigemina Infections of Cattle in Philippines
Yu, L; Terkawi, MA; Cruz-Flores, MJ; Claveria, FG; Aboge, GO; Yamagishi, J; Goo, YK; Cao, S; Masatani, T; Nishikawa, Y; Xuan, X (2013)A total of 250 blood samples were collected from clinically healthy cattle in five provinces of Philippines. DNA was extracted from the samples and analyzed by nested PCR assays for an epidemiological survey of Babesia ... -
Identification and characterization of an interspersed repeat antigen of Babesia microti (BmIRA)
Cao, S; Luo, Y; Aboge, GO; Terkawi, MA; Masatani, T; Suzuki, H; Igarashi, I; Nishikawa, Y; Xuan, X (2013)In this report, a novel gene encoding an interspersed repeat antigen from Babesia microti (BmIRA) was identified and described. The full-length cDNA containing an open reading frame of 1,947 bp was obtained by immunoscreening ... -
Identification of an immunodominant Babesia gibsoni 47-kDa antigen
Goo, Y-K; Jia, H; Aboge, G O; Terkawi, M A; Yamagishi, J; Nishikawa, Y; Igarashi, I; Xuan, X (Department of Public Health, Pharmacology and Toxicology, 2009)The inosine monophosphate dehydrogenase(IMPDH )enzyme has been characterized and validate dasa molecular drug target in other apicomplexans butnot in the genus Babesia. Subsequently,we cloned and expressed a Babesia ... -
Molecular and immunological characterization of Babesia gibsoni
Terkawi, M Alaa; Aboge, G; Jia, H; Goo, Y-K; Ooka, H; Yamagishi, J; Nishikawa, Y; Yokoyama, N; Igarashi, I; Kawazu, S-I; Fujisaki, K; Xuan, X (Department of Public Health, Pharmacology and Toxicology, 2009)Serological immune screening was used to identify a gene encoding heat shock protein-70 from Babesia gibsoni (BgHSP-70) that showed high homology with HSP-70s from other apicomplexan parasites. This gene corresponded to a ... -
Molecular characterization of a novel 32-kDa merozoite antigen of Babesia gibsoni with a better diagnostic performance by enzyme-linked immunosorbent assay
Aboge, G O; Jia, H; Kuriki, H; Zhou, J; Nishikawa, Y; Igarashi, I; Fujisaki, K; Suzuki, H; Xuan, X (Department of Public Health, Pharmacology and Toxicology, 2007)We cloned and expressed 3 novel gene encoding a 32-kDa merozoite protein of Babesia gibsoni (BgP32). The length of nucleotide sequence of the cD;\' A \\'3. 1-1-6-1 bp with an open reading frame of 969 bp. The truncated ... -
Molecularand immunological characterization of Babesia gibsoni and Babesia microti heat shockprotein-70
Terkawi, M A; Jia, H J; Goo, Y-K; Ooka, H; Yamagishi, J; Nishikawa, Y.; Igarashi, I; Fujisaki, K; Xuan, X (Department of Public Health Pharmacology and Toxicology, 2009)Serological immunoscreening was used to identify a gene encoding heat shock protein-70 from Babesia gibsoni (BgHSP-70) that showed high homology with HSP-70s from other apicomplexan parasites. This gene corresponded to ... -
Precursors of methotrexate target dihydrofolate reductase-thymidylate synthase of Babesia gibsoni
Aboge, G O; Terkawi, M; Goo, Y-K; Batbaatar, V; Nishikawa, Y; Sunaga, F; Namikawa, K; I, Igarashi; Fujisaki, K; Suzuki, H; Xuan, X (Department of Public Health Pharmacology and Toxicology, 2010)It is not known whether precursors of methotrexate, such as 2, 4-diamino-6-hydroxymethyl-pteridine (DAP) and 2, 4-diamino-N10-methyl-pteroic acid (DAMPA), could target the dihydrofolate reductase-thymidylate synthase ... -
Seroprevalence of Cryptosporidium parvum infection of dairy cows in three northern provinces of Thailand determined by enzyme-linked immunosorbent assay using recombinant antigen CpP23
Inpankaew, T; Jittapalapong, S; Phasuk, J; Pinyopanuwut, N; Chimnoi, W; Kengradomkit, C; Sunanta, C; Zhang, G; Aboge, G.O; Nishikawa, Y; Igarashi, I; Xuan, X (2009)Cryptosporidium parvum is the most frequent parasitic agent that causes diarrhoea in AIDS patients in Thailand. Cryptosporidiosis outbreaks in humans may be attributed to contamination of their drinking water from infected ... -
TgGRA23, a novel Toxoplasma gondii dense granule protein associated with the parasitophorous vacuole membrane and intravacuolar network
Masatani, T; Matsuo, T; Tanaka, T; Terkawi, MA; Lee, EG; Goo, YK; Aboge, GO; Yamagishi, J; Hayashi, K; Kameyama, K; Cao, S; Nishikawa, Y; Xuan, X (2013)Toxoplasma gondii is an intracellular protozoan parasite, which relies on a specialized compartment, the parasitophorous vacuole (PV), to survive within host cells. Dense granules within the parasite release a large variety ... -
The use of a recombinant baculovirus expressing a chitinase from the hard tick Haemaphysalis longicornis and its potential application as a bioacaricide for tick control
Assenga, SP; You, M; Shy, CH; Yamagishi, J; Sakaguchi, T; Zhou, J; Kibe, MK; Xuan, X; Fujisaki, K (College of Health Sciences, University of NairobiNational Research Center for Protozoan Disease, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan, 2006)Baculoviruses are specific insect pathogens used as selective biological insecticides on lepidopteran insects. We have tested a recombinant baculovirus expressing a chitinase gene for its efficacy as a tick bioacaricide. ...