The selection of a standard STR panel for DNA profiling of the African elephant (Loxodonta africana) in Kenya
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Date
2014-10-26Author
Kinuthia, Julius
Harper, Cindy
Muya, Shadrack
Kimwele, Charles
Alakonya, Amos
Muigai, Anne
Gakuya, Francis
Mwaniki, Mercy
Gatebe, Erastus
Language
enMetadata
Show full item recordAbstract
Abstract The African elephant (Loxodonta africana)
population in Kenya is declining at an alarming rate due to
habitat destruction, human-elephant conflicts and the current
escalation of poaching for ivory. This study established
a standard protocol for forensic analysis of L.
africana and their products such as ivory. Three multiplex
PCR panels consisting of 17 Short Tandem Repeat markers
were selected from 40 markers using bioinformatics tools,
amplification and polymorphism. Genotyping was successful
and reproducible. This method is efficient, accurate
and cost effective and has potential for application in
conservation of L. africana.
Citation
Kinuthia, J., Harper, C., Muya, S., Kimwele, C., Alakonya, A., Muigai, A., ... & Gatebe, E. (2014). The selection of a standard STR panel for DNA profiling of the African elephant (Loxodonta africana) in Kenya. Conservation Genetics Resources, 1-3.Publisher
University of Nairobi