A cross-sectional study on the prevalence of subclinical mastitis and antimicrobial susceptibility patterns of the bacterial isolates in milk samples of smallholder dairy goats in Kenya
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Date
2014Author
Mbindyo, C M
Gitao, C G
Bebor, L.
Language
enMetadata
Show full item recordAbstract
Dairy goa
t production is an emerging enterprise
, which has a lot of potenti
al for poverty
alleviation, improved nutrition, and increased income for the poor and can play a role in
contribution towards Kenya’s development plan. Inadequate information on prevalence of
subclinical mastitis and antibiotic sensitivity are some of the
challenges facing this industry.
This study was carried out in dairy goats under zero grazing system in Mount Kenya region,
from January 2012 to December 2012 to determine the prevalence of subclinical mastitis in
lactating goats and the antibiotic sensit
ivity of the isolated bacteria.
A total of 310 lactating goats were randomly selected from populations in
Meru Nyeri and Embu
counties and screened for bacterial carriage, as evidence of subclinical mastitis. Six hundred and
twenty (620) milk samples from
the 310 goats (right and left quarters) were aseptically collected;
first screened using California Mastitis Test (CMT), then cultured for bacterial isolation and
characterization. Antibiotic sensitivity testing was also performed on the isolated bacteri
a American Journal of Research Communication
www.usa
-
journals.com
Mbindyo,
et al
., 2014: Vol 2(8)
31
ajrc.journal@gmail.com
Based on culture results, the prevalence of subclinical mastitis was 59% in Meru County, 58% in
Embu County and 54 % in Nyeri County. An overall mean prevalence of 57% was estimated in
the three counties. There was no significant difference in subclini
cal mastitis prevalence in the
three counties (P=0.75). Based on CMT, the prevalence of subclinical mastitis was estimated to
be 61% in Meru County, 61% in Embu and 60% in Nyeri County. The overall mean prevalence
was estimated to be 61%.There was no signi
ficance difference between prevalence of subclinical
mastitis in the three counties (P=0.96).
Among the 620 milk samples collected from the 310 lactating goats, 317 (51%) were California
mastitis test positive, and on culturing, 304 (96%) yielded bacterial
growth. The following
bacteria were isolated from the milk samples;
Coagulase Negative
Staphylococcus
was the
most
prevalent
-
at 2
8
.3% (176/
6
20
), followed by
Staphylococcus aureus
-
at 1
3
.5% (84/
6
20
),
Streptococcus
-
at
8.8% (46/
6
20
),
Escherichia coli
-
at
3% (19/
6
20
),
Micrococcus
-
at 4%
(24/
6
20
),
Corynebacterium
-
at 1% (7/
6
20
),
Pseudomonas
-
at 0.1% (1/
6
20
).
Of the
Streptococcus
isolates, 1.5% (9/
6
20
) were
Streptococcus agalactiae.
Norfloxacin and gentamycin were antibiotics that the organisms were m
ost sensitive to while
kanamycin and amoxycillin were antibiotics that the organisms were least sensitive to.
The study revealed that there is high prevalence of subclinical mastitis in dairy goats in Mount
Kenya region.
The high prevalence of
subclinical
mastitis
recorded in this study has a negative
impact in dairy goat productivity
;
and
there is, therefore, need to create awareness on
the
problem with a view to instituting
appropriate cont
rol measures
. The study also revealed that
CMT is a reliable test
for subclinical mastitis in goats. Since it is easy to carry out, rapid and cheap, it is recommended that goat associations make use of it as part of the control measures;
they can train specific personnel to carry out and interpret the test.
Keywords:
An
tibiotic susceptibility, Bacterial pathogens, Dairy goats, Subclini
cal Mastitis,
prevalence, Kenya
Citation
C. M Mbindyo, C.G Gitao, L. Bebora . A cross - sectional study on the prevalence of subclinical mastitis and antimicrobial susceptibility patterns of the bacterial isolates in milk samples of smallholder dairy goats in Kenya . American Journal of Research Communication, 2014, 2(8): 3 0 - 51 } www.usa - journals.com , ISSN: 2325 - 4076.Publisher
University of Nairobi