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Bovine granulocyte/macrophage and erythroid colony culture: characteristics of the colonies and the assay systems
(University of Nairobi., 1979-10)
Bovine bone marrow granulocyte/macrophage colonies were cultured in vitro in methyl cellulose and in plasma clots using bovine endotoxin-stimulated serum as a source of colony stimulating activity. The endotoxin-stimulated ...
Inhibition of leukopoiesis by sera from Trypanosoma congolense infected calves: partial characterization of the inhibitory factor
(University of Nairobi., 1980-06)
Sera collected from calves infected with Trypanosoma congolense inhibited bovine granulocyte/macrophage colony formation in vitro, but not erythroid colony formation. The inhibitor was found to be TCA precipitable in nature ...
The kinetics of haemopoietic differentiation assessed by in vitro bone marrow culture in endotoxin-treated and in anaemic calves
(University of Nairobi., 1980-07)
Marrow aspirates from calves injected with Pseudomonas aeruginosa endotoxin showed an increased number of granulocyte/macrophage progenitor cells, and a reduced number of erythroid progenitor cells. On the other hand, ...
Characteristics of erythroid colonies formed by bovine marrow progenitor cells in methyl cellulose cultures
(University of Nairobi., 1982-03)
Bovine erythroid colonies containing eight to 100 erythroid cells were successfully cultured in methyl cellulose. The colonies required four to five days to mature and a direct linear relationship (r = 0.99) between the ...
Inhibition of bovine bone marrow granulocyte/macrophage colony formation in vitro by serum collected from cattle infected with Trypanosoma vivax or Trypanosoma congolense
(University of Nairobi., 1979-06)
Serum collected from cattle infected with Trypanosoma vivax or Trypanosoma congolense inhibited bovine granulocyte/macrophage colony formation in methyl cellulose cultures. Maximum inhibition was caused by serum collected ...
Cryopreservation of bovine hemopoietic progenitor cells in liquid nitrogen
(University of Nairobi., 1981-04)
Bovine marrow granulocyte/macrophage and erythroid progenitor cells maintained viability after storage in liquid nitrogen for 2 to 4 weeks. The granulocyte/macrophage progenitor cells maintained 100% viability for 4 weeks, ...