dc.contributor.author | Magomere, T O | |
dc.contributor.author | Ngugi, K | |
dc.contributor.author | Mutitu, E | |
dc.contributor.author | Shibairo, S | |
dc.contributor.author | Obukosia, S | |
dc.date.accessioned | 2015-02-17T10:05:40Z | |
dc.date.available | 2015-02-17T10:05:40Z | |
dc.date.issued | 2014 | |
dc.identifier.citation | Magomere TO, Ngugi K, Mutitu E, Shibairo S, Obukosia S. "Real Time PCR Mediated Determination Of The Spontaneous Occurrence Of Sorghum bicolor (L. Moench) Alleles In Wild Sorghum Populations." African Journal of Biotechnology . 2014. | en_US |
dc.identifier.uri | http://hdl.handle.net/11295/80368 | |
dc.description.abstract | The study evaluates the utility of Real Time PCR (RT
-
PCR) in quantitative and qualitative analysis of
alleles in sorghum populations and the spontaneous occurrence of
Sorghum
bicolor
alleles in wild
populations of sorghum. Leaf and seed material from wild
sorghum accesions were sampled in
Homabay, Siaya and Busia counties to represent Western Kenya sorghum producing regions. A second
sampling was done on S
2
populations of
S
.
bicolor
,
Sorghum
halepense
and
Sorghum
sudanense
maintained in the greenhouse.
Crop loci were evaluated in all materials using a LightCycler® 2.0
system. Real Time PCR was effective in qualitative and quantitative determination of crop alleles in both
crop and weedy backgrounds of
S. sudanense
,
S. halepense
and
S. verticilliflorum.
C
rossing point
values ranged between 19.7 from 30 ng template to 35.9 from 0.015 pg of template on locus SB1764.
Melting peaks analysis ranged between 83.29 to 88°
C
on locus SB1764 and between 86.01 to 80.88°
C
on
locus SB3420 effectively differentiating the
4 species. RealTime
-
PCR was successful in quantitative and
qualitative analysis of specific crop alleles from loci SB1764 and SB3420 from seed and leaf DNA.
Spontaneous occurrence of crop and rare alleles in wild sorghum populations growing in sympatry wi
th
crop cultivars showed the presence of crop and rare alleles in wild sorghum populations. Means of wild
populations from lower midland
1
(LM
1
), LM
2
, LM
3
and LM
4
AEZs were not significantly different. It is
therefore
vital
to
test
S.
bicolor
seeds
and
other
plant
materials
in
transit,
at
entry
points
and
populations
of growing plants for foreign genes including transgenes using RT
-
PCR | |
dc.language.iso | en | en_US |
dc.publisher | University of Nairobi | en_US |
dc.title | Real time pcr mediated determination of the spontaneous occurrence of sorghum bicolor (l. Moench) alleles in wild sorghum populations | en_US |
dc.type | Article | en_US |
dc.type.material | es | en_US |