In-vitro anthelmintic bioactivity study of eclipta prostrata l. (whole plant) using adult haemonchus contortus worms: a case study of Migori County, Kenya

Abstract

The aim of this study was to investigate the anthelmintic activity of Eclipta prostrata (Asteraceae) which is used by traditional medicine practitioners in Migori County using adult Haemonchus contortus worm as a model.50gm of ground powder of Eclipta prostrata (whole plant) was extracted separately with 300 ml methanol, acetone and water. The yields of the extracts were 3.53 gm, 4.19 gm and 4.02 gm for methanol, acetone and water respectively. The anthelmintic activity of 6.25 mg/ml, 12.5 mg/ml and 25 mg/ml concentrations of aqueous, acetone and methanol crude extracts of Eclipta prostrata (whole plant), were compared with the effect produced by the standard reference drug albendazole with Phosphate Buffered Saline (PBS) used as a negative control. Death of Haemonchus contortus worm was determined within a period of 24 hrs. Eclipta prostrata extract had mean mortality of 26.7-40.0 % at 6.25 mg/ml; 33.3-53.3 % at 12.5 mg/ml; 36.7-56.7 % at 25 mg/ml.All the extracts contained tannins and cardiac glycosides