| dc.description.abstract | Epidemiological surveillance of porcine group A rotavirus (RVA) strains was conducted in 5 swine herds in Ohio, USA using historical (2004) and recent (2011-2012) fecal samples. Of the 371 samples examined, 9.4% (35/371) were positive for RVA. The RVA detection rates increased from 5.9% in 2004, 8.5% in 2011 to 13.8% in 2012. A total of 23 positive samples were analyzed for RVA G and P genotypes. The dominant G-P combination was G9P[13] found in 60.9% of positive samples. The other combinations were G9P[7] (8.7%), G4P[13] (8.7%), G11P[13] (4.3%) and G11P[7] (4.3%). Sequence analysis of partial VP7 genes of selected strains revealed that the G4 strains were closely related to one another (95%) and to a lower extent to human (82-84%) and porcine (84-86%) G4 strains. The G11 strains detected shared identical VP7 gene sequence (100%) and were closely related to human (85-86%) and other porcine (83%) G11 strains. The G9 strains identified were closely related to one another, human and other porcine strains (96-97%, 89-91% and 89-91% nucleotide identities, respectively). The VP4 gene analysis revealed that P[7] strains were closely related to each other and to P[7] strains isolated from porcine, bovine and panda (91-99%, 92-99% and 92-99%, respectively). The P[13] strains showed a higher diversity among themselves and with other porcine P[13] strains ranging from 83% to 99% and 82-97%, respectively. Our results demonstrate broad genetic heterogeneity of the RVA strains and suggest the possibility of genetic reassortment between different RVA genotypes within these farms | en_US |
