| dc.contributor.author | Meyer, HHD | |
| dc.contributor.author | Eisele, K | |
| dc.contributor.author | Osaso, J | |
| dc.date.accessioned | 2015-08-04T13:40:03Z | |
| dc.date.available | 2015-08-04T13:40:03Z | |
| dc.date.issued | 1989 | |
| dc.identifier.citation | Prostaglandins Volume 38, Issue 3, September 1989, Pages 375-383 | en_US |
| dc.identifier.uri | http://www.sciencedirect.com/science/article/pii/0090698089901408 | |
| dc.identifier.uri | http://hdl.handle.net/11295/89533 | |
| dc.description.abstract | As an alternative for radioimmunoassays a new enzyme immunoassay (EIA) for the determination of 13, 14-dihydro-15-keto PGF2α (PGFM) has been developed. Biocytin was linked to PGFM by the N-hydroxysuccinimide method and the product (biocytinyl-PGFM) purified by reversed phase column chromatography. Biocytinyl-PGFM was used in the EIA as a bridge between the immobilized PGFM-antibody and streptavidin-peroxidase. The absolute sensitivity of the assay was about 160 amol (92% rel. binding) and required only 2 μl plasma for PGFM estimation within the whole physiological range (0.08–20 pmol/ml). All variabilities were < 14 %. The described assay procedure may be of general applicability for other prostaglandins. | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | University of Nairobi | en_US |
| dc.title | Biotin-streptavidin amplified enzymeimmunoassay for 13, 14-dihydro-15-keto-PGF | en_US |
| dc.type | Article | en_US |
| dc.type.material | en | en_US |
