• Login
    • Login
    Advanced Search
    View Item 
    •   UoN Digital Repository Home
    • Theses and Dissertations
    • Faculty of Science & Technology (FST)
    • View Item
    •   UoN Digital Repository Home
    • Theses and Dissertations
    • Faculty of Science & Technology (FST)
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Ethnobotanical, bioactivity and phytochemical evaluation of anthelmintic herbal remedies of Migori county, Kenya

    Thumbnail
    View/Open
    Full text (1.473Mb)
    Date
    2014
    Author
    Sirama, Victor Okello
    Type
    Thesis; en_US
    Language
    en
    Metadata
    Show full item record

    Abstract
    Intestinal worms affect a host of individuals resulting in malnutrition, stunted growth, intellectual retardation and cognitive deficits. The aim of this study was to investigate the indigenous plants of Migori County with anthelmintic activity using adult Haemonchus contortus worm as a model and determining the presence of active principles. Twenty six (26) herbalists between the ages 20-69 years (10 men and 16 women) were interviewed on plants used as anthelmintics. Local name, parts used, mode of preparation and administration were documented. There was a high correlation (r=0.96) between age of informants and number of medicinal plant citations. The study identified 21 anthelmintic plants distributed among 21 genera and 13 families. The plant families most commonly used includes Asteraceae (27.72%), Leguminosae (15.79%) and Lamiaceae (10.53%). The three most frequently used plants: Eclipta alba (Asteraceae), Vernonia amygdalina (Asteraceae) and Plectranthus barbatus (Lamiaceae) were collected, extracted in methanol, acetone and distilled water for phytochemical studies. The phytochemical screening focussed on saponins, tannins and cardiac glycosides using standard procedures. In-vitro anthelmintic activities study of crude extracts of these selected plants were also done using adult Haemonchus contortus worms to test their potency. The anthelmintic activity of 6.25 mg/ml, 12.5 mg/ml and 25 mg/ml concentrations of aqueous, acetone and methanol crude extracts of Eclipta alba (whole plant), leaf and root extracts of Vernonia amygdalina and leaf extract of Plectranthus barbatus were compared with the effect produced by the standard reference drug albendazole. Extracts at 25 mg/ml concentrations were most active. Phosphate Buffered Saline (PBS) was used as a negative control. Death was determined within a period of 24 hrs. Extracts tested were active against Haemonchus contortus except 6.25 mg/ml concentration of acetone extract of P. barbatus. Eclipa alba (whole plant) extract was the most potent followed by Vernonia amygdalina root extract. Plectranthus barbatus leaf extract was the least potent. Albendazole had an overall average MI of 1.00 which translates to 100% potency. Analysis of variance revealed that there were significant differences in the anthelmintic activity of the plants’ extracts of all the concentrations (6.25, 12.5 and 25 mg/ml) used at p 0.05. All the three plant extracts exhibited anthelmintic activity therefore could be a cheap and readily available alternative source of anthelmintic treatment.
    URI
    http://hdl.handle.net/11295/90331
    Citation
    Master of science in plant taxonomy and economic botany
    Publisher
    University of Nairobi
    Subject
    Eclipta alba
    Vernonia amygdalina
    Plectranthus barbatus
    Haemonchus contortus
    Phytochemicals
    In-vitro anthelmintic activity
    Albendazole
    Migori County
    Kenya
    Collections
    • Faculty of Science & Technology (FST) [3948]

    Copyright © 2022 
    University of Nairobi Library
    Contact Us | Send Feedback

     

     

    Useful Links
    UON HomeLibrary HomeKLISC

    Browse

    All of UoN Digital RepositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Copyright © 2022 
    University of Nairobi Library
    Contact Us | Send Feedback