Canine distemper virus neutralization activity is low in human serum and it is sensitive to an amino acid substitution in the hemagglutinin protein
Date
2015Author
Zhanga, Xinsheng
Wallace, Olivia L
Domi, Arban
Wright, Kevin J
Driscolla, Jonathan
Anzala, Omu
Sanders, Eduard J
Kamalie, Anatoli
Karita, Etienne
Alleng, Susan
Fasth, Pat
Gilmouri, Jill
Price, Matt A
Parks, Christopher L
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Serum was analyzed from 146 healthy adult volunteers in eastern Africa to evaluate measles virus (MV) and canine distemper virus (CDV) neutralizing antibody (nAb) prevalence and potency. MV plaque reduction neutralization test (PRNT) results indicated that all sera were positive for MV nAbs. Furthermore, the 50% neutralizing dose (ND50) for the majority of sera corresponded to antibody titers induced by MV vaccination. CDV nAbs titers were low and generally were detected in sera with high MV nAb titers. A mutant CDV was generated that was less sensitive to neutralization by human serum. The mutant virus genome had 10 nucleotide substitutions, which coded for single amino acid substitutions in the fusion (F) and hemagglutinin (H) glycoproteins and two substitutions in the large polymerase (L) protein. The H substitution occurred in a conserved region involved in receptor interactions among morbilliviruses, implying that this region is a target for cross-reactive neutralizing antibodies
URI
http://www.sciencedirect.com/science/article/pii/S0042682215001750http://hdl.handle.net/11295/95910
Citation
Virology Volume 482, August 2015, Pages 218–224Publisher
Elsevier
Subject
Canine distemper virusMeasles virus
Plaque reduction neutralization test
Neutralizing antibody
Hemagglutinin
Pre-existing immunity
Cross-neutralization
Rights
Attribution-NonCommercial-ShareAlike 3.0 United StatesUsage Rights
http://creativecommons.org/licenses/by-nc-sa/3.0/us/Collections
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