dc.contributor.author | Wadegu, Meshack | |
dc.contributor.author | Wamunyokoli, Fred | |
dc.contributor.author | Osanjo, George | |
dc.contributor.author | Opanda, Silvanos | |
dc.contributor.author | Majanja, Janet | |
dc.contributor.author | Rodney, Coldren | |
dc.contributor.author | Bulimo, Wallace | |
dc.date.accessioned | 2017-03-16T07:52:04Z | |
dc.date.available | 2017-03-16T07:52:04Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Wadegu, Meshack, et al. "Molecular Surveillance of Adamantane Resistance among Human Influenza A Viruses Isolated in Four Epidemic Seasons in Kenya." African Journal of Pharmacology and Therapeutics 5.3 (2016). | en_US |
dc.identifier.uri | http://journals.uonbi.ac.ke/ajpt/article/view/1533 | |
dc.identifier.uri | http://hdl.handle.net/11295/100560 | |
dc.description.abstract | Background: Adamantanes impede influenza A virus replication and are important in the treatment and prophylaxis of disease caused by these viruses. Genotypic characterization of influenza A viruses for mutations associated with resistance to adamantanes has not been fully investigated in Kenya.
Objective: To characterize susceptibility of influenza A virus subtypes that circulated in Kenya from 2008-2011 to adamantanes.
Methods: Archived influenza A virus strains obtained from 2008 to 2011 were propagated in MDCK cells prior to sequencing of the matrix and hemagglutinin gene segments, followed by bioinformatics analyses.
Results: Ninety two virus strains consisting of 21 A/H3N2, 18 A/H1N1 and 53 A/H1N1pdm09 were analyzed. All A/H3N2 and A/H1N1pdm09 viruses displayed resistance to adamantanes due to the S31N/S31D amino acid substitution. All A/H1N1pdm09 virus strains belonged to the N-lineage characterized by S203T amino acid substitution in the HA1. All A/H1N1 viruses were sensitive to adamantane and were characterized by K140E amino acid substitution in the HA1.
Conclusion: All Kenyan influenza A/H3N2 and A/H1N1pdm09 virus strains were resistant to adamantanes while seasonal A/H1N1 strains were sensitive to these drugs. During the study period, Amantadine and Rimantadine were inappropriate for prophylaxis and treatment of influenza disease caused by A/H3N2 and A/H1N1pdm09 virus subtypes in Kenya. | en_US |
dc.language.iso | en | en_US |
dc.publisher | University of Nairobi | en_US |
dc.rights | Attribution-NoDerivs 3.0 United States | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nd/3.0/us/ | * |
dc.subject | Kenya, influenza A/H3N2, A/H1N1pdm09, A/H1N1, adamantanes | en_US |
dc.title | Molecular surveillance of adamantane resistance among human influenza a viruses isolated in four epidemic seasons in Kenya | en_US |
dc.type | Article | en_US |