The impact of sex work interruption on blood-derived t cells in sex workers from Nairobi, Kenya
Date
2016Author
Omollo, Kenneth
Geneviève, Boily-Larouche
Julie, Lajoie
Kimani, Makobu
Julianna, Cheruiyot
Kimani, Joshua
Oyugi, Julius
Fowke, Keith R
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Background: Unprotected sexual intercourse exposes the female genital tract (FGT) to semen-derived antigens, which leads to a proinflammatory response. Studies have shown that this postcoital inflammatory response can lead to recruitment of activated T cells to the FGT, thereby increasing risk of HIV infection.
Objective: The purpose of this study was to evaluate the impact of sex work on activation and memory phenotypes of peripheral T cells among female sex workers (FSW) from Nairobi, Kenya.
Subjects: Thirty FSW were recruited from the Pumwani Sex Workers Cohort, 10 in each of the following groups: HIV-exposed seronegative (at least 7 years in active sex work), HIV positive, and New Negative (HIV negative, less than 3 years in active sex work). Blood was obtained at three different phases (active sex work, abstinence from sex work–sex break, and following resumption of sex work). Peripheral blood mononuclear cells were isolated and stained for phenotypic markers (CD3, CD4, CD8, and CD161), memory phenotype markers (CD45RA and CCR7), activation markers (CD69, HLA-DR, and CD95), and the HIV coreceptor (CCR5). T-cell populations were compared between groups.
Results: In HIV-positive women, CD8+CCR5+ T cells declined at the sex break period, while CD4+CD161+ T cells increased when returning to sex work. All groups showed no significant changes in systemic T-cell activation markers following the interruption of sex work, however, significant reductions in naive CD8+ T cells were noted. For each of the study points, HIV positives had higher effector memory and CD8+CD95+ T cells and lower naive CD8+ T cells than the HIV-uninfected groups.
Conclusions: Interruption of sex work had subtle effects on systemic T-cell memory phenotypes.
Citation
Omollo, Kenneth, et al. "The Impact of Sex Work Interruption on Blood-Derived T Cells in Sex Workers from Nairobi, Kenya." AIDS research and human retroviruses 32.10-11 (2016): 1072-1078.Publisher
University of Nairobi
Rights
Attribution-NonCommercial-NoDerivs 3.0 United StatesUsage Rights
http://creativecommons.org/licenses/by-nc-nd/3.0/us/Collections
- Faculty of Health Sciences (FHS) [10387]
The following license files are associated with this item: