Isolation of erythromycins and related substances from fermentation residues of strepto.'i1yces erythreus by high-performance liquid chromatography on silica gel

dc.contributor.author	Kibwage, IO
dc.contributor.author	Janssen, G
dc.contributor.author	Roets, E
dc.contributor.author	Hoogmartens, J
dc.contributor.author	Vanderhaeghe, H
dc.date.accessioned	2013-02-19T08:51:57Z
dc.date.issued	1985
dc.identifier.citation	Journal of Chromatogr aphv, 346 (1985) 309-319	en
dc.identifier.uri	http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/10225
dc.description.abstract	Preparative high-performance liquid chromatography on silica gel allows the isolation of erythromycins and related substances from mother-liquor concentrates. Three mobile phases were used consecutively: A, ethyl acetate-methanol-25% am¬monia (100:8:1, v.v); B, diethyl ether-methanol-25% ammonia (l00:7:1. v,/v) and C, dichloromethane-methanol-25% ammonia (lOO:5:0,5, \i'v). The separation and puri¬fication was confirmed by thin-layer chromatography. Thirteen pure substances were isolated among which are erythromycins A, B, C and D, 8,9-anhydroerythromycin A-6,9-hemiketal, erythromycins A and C-6,9;6,12-spiroketals and N-demethylery¬thromycin A-6.9:9,12-spiroketal.	en
dc.language.iso	en	en
dc.title	Isolation of erythromycins and related substances from fermentation residues of strepto.'i1yces erythreus by high-performance liquid chromatography on silica gel	en
dc.type	Article	en