Isolation of erythromycins and related substances from fermentation residues of strepto.'i1yces erythreus by high-performance liquid chromatography on silica gel
dc.contributor.author | Kibwage, IO | |
dc.contributor.author | Janssen, G | |
dc.contributor.author | Roets, E | |
dc.contributor.author | Hoogmartens, J | |
dc.contributor.author | Vanderhaeghe, H | |
dc.date.accessioned | 2013-02-19T08:51:57Z | |
dc.date.issued | 1985 | |
dc.identifier.citation | Journal of Chromatogr aphv, 346 (1985) 309-319 | en |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/10225 | |
dc.description.abstract | Preparative high-performance liquid chromatography on silica gel allows the isolation of erythromycins and related substances from mother-liquor concentrates. Three mobile phases were used consecutively: A, ethyl acetate-methanol-25% am¬monia (100:8:1, v.v); B, diethyl ether-methanol-25% ammonia (l00:7:1. v,/v) and C, dichloromethane-methanol-25% ammonia (lOO:5:0,5, \i'v). The separation and puri¬fication was confirmed by thin-layer chromatography. Thirteen pure substances were isolated among which are erythromycins A, B, C and D, 8,9-anhydroerythromycin A-6,9-hemiketal, erythromycins A and C-6,9;6,12-spiroketals and N-demethylery¬thromycin A-6.9:9,12-spiroketal. | en |
dc.language.iso | en | en |
dc.title | Isolation of erythromycins and related substances from fermentation residues of strepto.'i1yces erythreus by high-performance liquid chromatography on silica gel | en |
dc.type | Article | en |
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