dc.contributor.author | Gibbs, A | |
dc.contributor.author | Buggert, M | |
dc.contributor.author | Edfeldt, G | |
dc.contributor.author | Ranefall, P. | |
dc.contributor.author | Introini, A | |
dc.contributor.author | Cheuk, S | |
dc.contributor.author | Martini, E | |
dc.contributor.author | Eidsmo, L | |
dc.contributor.author | Ball, TB | |
dc.contributor.author | Kimani, J | |
dc.contributor.author | Kaul, R | |
dc.contributor.author | Karlsson, A | |
dc.contributor.author | Wählby, C | |
dc.contributor.author | Broliden, K | |
dc.contributor.author | Tjernlund, A | |
dc.date.accessioned | 2018-01-15T11:36:48Z | |
dc.date.available | 2018-01-15T11:36:48Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | 10.1093/infdis/jix661. | en_US |
dc.identifier.uri | https://www.ncbi.nlm.nih.gov/pubmed/29272532 | |
dc.identifier.uri | http://hdl.handle.net/11295/102352 | |
dc.description.abstract | BACKGROUND:
Genital mucosa is the main portal of entry for various incoming pathogens, including HIV, hence an important site for host immune defenses. Tissue-resident memory T (TRM) cells defend tissue barriers against infections and are characterized by expression of CD103 and CD69. Here we describe the composition of CD8+ TRM cells in the ectocervix of healthy and HIV-infected women.
METHODS:
Study samples were collected from healthy Swedish and Kenyan HIV-infected and uninfected women. Customized computerized image-based in situ analysis was developed to assess the ectocervical biopsies. Genital mucosa and blood samples were assessed by flow cytometry.
RESULTS:
While ectocervical epithelium of healthy women was populated with bona fide CD8+ TRM cells (CD103+CD69+), HIV-infected women displayed high frequency of CD103-CD8+ cells residing close to their epithelial basal membrane. Accumulation of CD103-CD8+ cells was associated with chemokine expression in the ectocervix and HIV viral load. CD103+CD8+ and CD103-CD8+ T cells expressed cytotoxic effector molecules in the ectocervical epithelium of healthy and HIV-infected women. Additionally, HIV-infected women had decreased frequencies of circulating CD103+CD8+ T cells.
CONCLUSIONS:
Our data provide insight into the distribution of CD8+ TRM cells in human genital mucosa, a critically important location for immune defense against pathogens, including HIV. | en_US |
dc.language.iso | en | en_US |
dc.publisher | University of Nairobi | en_US |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 United States | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/us/ | * |
dc.subject | CD8+ TRM; HIV; genital mucosa; imaging analysis; in situ | en_US |
dc.title | HIV-infected women have high numbers of CD103-CD8+ T cells residing close to the basal membrane of the ectocervical epithelium. | en_US |
dc.type | Article | en_US |